General description

Cathepsin B (CTSB; EC 3.4.22.1) is a lysosomal cysteine proteinase involved in intracellular degradation and turnover of proteins. It is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP is a causative factor in Alzheimer′s disease. CTSB has also been implicated in the invasion and metastasis of certain cancers. The Cathepsin B Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSB. CTSB activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (λ_ex = 400/λ_em = 505 nm), proportional to the enzymatic activity present.

Principle

The Cathepsin B Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSB. CTSB activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (λ_ex = 400/λ_em = 505 nm), proportional to the enzymatic activity present.

Suitability

Suitable for the screening of inhibitors of Cathepsin B.